Note: PDF files are for use by students for personal copies only

Bacterial populations and pathogenesis

1. Thursday, January 15, 2004. What is a pathogen?

Assigned article:

Paster, B. J., S. K. Boches, J. L. Galvin, R. E. Ericson, C. N. Lau, V. A. Levanos, A. Sahasrabudhe, and F. E. Dewhirst. 2001. Bacterial diversity in human subgingival plaque. J Bacteriol 183:3770-3783. PDF (LARGE! 8.9 MB)

Powerpoint Presentation

2. Tuesday, January 20, 2004. Microarrays as a global approach to pathogenesis and gene expression.

Assigned article:

Lorenz, M. C., and G. R. Fink. 2001. The glyoxylate cycle is required for fungal virulence. Nature 412:83-6. PDF (210 KB)

Related article:

Lorenz, M. C., and G. R. Fink. 2002. Life and death in a macrophage: role of the glyoxylate cycle in virulence. Eukaryot. Cell 1:657-62. PDF (104 KB)

3. Thursday, January 22, 2004. Identifying virulence factors/genes I

Assigned article:

Hang L, John M, Asaduzzaman M, Bridges EA, Vanderspurt C, Kirn TJ, Taylor RK, Hillman JD, Progulske-Fox A, Handfield M, Ryan ET, Calderwood SB. 2003. Use of in vivo-induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibrio cholerae. Proc Natl Acad Sci U S A. 100(14): 8508-13. PDF (327 KB)

Useful Review:

Angelichio MJ, Camilli A. 2002. In vivo expression technology. Infect Immun. 70(12): 6518-23. PDF (108 KB)

4. Tuesday, January 27, 2004. Identifying virulence factors/genes II

Merrell DS, Hava DL, Camilli A. 2002. Identification of novel factors involved in colonization and acid tolerance of Vibrio cholerae. Mol Microbiol. 43(6): 1471-91. PDF (1027 KB)

Useful Review:

Mecsas J. 2002. Use of signature-tagged mutagenesis in pathogenesis studies. Curr Opin Microbiol. 5(1): 33-7. PDF (83 KB)

5. Thursday, January 29, 2004. Identifying virulence factors/genes II

Assigned Article:

Shimono N, Morici L, Casali N, Cantrell S, Sidders B, Ehrt S, Riley LW. 2003. Hypervirulent mutant of Mycobacterium tuberculosis resulting from disruption of the mce1 operon. Proc Natl Acad Sci U S A. 100(26): 15918-23. PDF (577 KB)

Useful Review:

Foreman-Wykert AK, Miller JF. 2003. Hypervirulence and pathogen fitness. Trends Microbiol. 11(3): 105-8. (Comment in: Trends Microbiol. 2003. 11(9):413-4). PDF (85 KB) PDF (58 KB)