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Jeffrey K. Actor, Ph.D. Address and Contact: |
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Research Summary | Lectures | Publications | Histology Resources | Links |
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![[Actor photo]](actor.gif)
Research Interests: Examination of host immune responses during parasitic disease; understanding proinflammatory and regulatory cytokines during mycobacterial infections; quantitative bioluminescent RT-PCR; vaccine development.
Research Summary: Our research interests are directed at understanding the host immune regulation of cytokines and proinflammatory response during parasitic infections. We developed and characterized models of tuberculosis in susceptible and resistant mice using modified MTB organisms. It is our hope that understanding the host response to infectious agents will allow development of effective vaccines and therapeutics to combat these diseases. We have a strong collaboration with investigators in the Department of Pathology.
The laboratory goals also include development of bovine and human Lactoferrin as a vaccine adjuvnat to augment cell mediated immunity. Specifically, Lactoferrin as an adjuvant with BCG protects alveolar integrity upon infection with Mycobacterium tuberculosis. The understanding of molecular mechanisms underlying Lactoferrin effects are of great interest, including its use as an immunomodulatory agent to control inflammatory processes.
To assist our goals, we have also developed a bioluminescent quantitative RT-PCR assay to reliably measure message for cytokines in small tissue samples. We utilized the great sensitivity of aequorin, a flash-type bioluminescent tag, to develop highly accurate, rapid and sensitive (attomols) quantitative PCR and RT-PCR assay to be used in a 96-well plate format. This procedure has enable us to examine in vivo events during pathogenesis.
Additional research involves the use of novel non-ionic blockcopolymers (poloxamers) as adjuvants to direct immune responses following vaccination. These adjuvants specifically influence the development of T helper, CTL and B cell responses when administered.
The use of these materials is
solely for teaching purposes!
MSI Immunology: How the Immune System Works Lecture 01/08/08
MSI Immunology: Cells and Organs of the Immune System Lecture 01/8/08
MSI Immunology: Innate Immunity Lecture 01/15/08
MSI Immunology: T Cell Receptor: Structure and Genetic Basis Lecture 02/5/08
MSI Immunology: Role of MHC Lecture 02/5/08
MSI Immunology: Activation of B and T cells Lecture 02/22/08
MSI Immunology: Cytokines Lecture 02/28/08
MSI Immunology: Infection and Immunity Lecture 03/27/07
MSI Immunology: Tolerance and Immune Regulation Lecture 04/10/07
MSI Immunology: Final Review Lecture 05/01/07
MSI Spring 2007-2008 Medic
Immunology Web Page
Hwang SA, Wilk KM, Budnicka M, Olsen M, Bangale YA, Hunter RL, Kruzel ML, Actor, J.K. (2007) Lactoferrin enhanced efficacy of the BCG vaccine to generate host protective responses against challenge with virulent Mycobacterium tuberculosis. Vaccine. 2007 Sep;(37-38):6730-43. Abstract
Hunter, R.L., Jagannath, C., Actor, J.K. (2007) Pathology of Postprimary Tuberculosis in Humans and Mice Tuberculosis: Contradiction of long-held beliefs. Tuberculosis (Edinb). 2007 Jul;87(4):267-78.Abstract
Kruzel, M.L., Actor, J.K., Boldogh,
Guidry, T.V., Hunter, R.L., Actor, J.K. (2007) Mycobacterial Glycolipid Trehalose 6,6’-Dimycolate Induced Hypersensitive Granulomas: Contribution of CD4+ Lymphocytes. Microbiology-UK(SGM). 2007; Oct;153(Pt 10):3360-9. Abstract
Hwang, S-A., Wilk, K.M., Budnicka, M., Olsen, M., Bangale, Y.A., Hunter, R.L., Kruzel, M.L., Actor, J.K. (2007) Lactoferrin enhanced efficacy of the BCG vaccine to generate host protective responses against challenge with virulent Mycobacterium tuberculosis. Vaccine. 2007; 17;25(37-38):6730-43. Abstract
Hwang, S.-A., Wilk, K.M., Bangale, Y.A., Kruzel, M.L., Actor, J.K. (2007) Lactoferrin Modulation of IL-12 and IL-10 Response from Activated Murine Leukocytes. Medical Microbiology and Immunology. 2007 Sep;196(3):171-80.Abstract
Hunter RL, Olsen MR, Jagannath C, Actor, JK. (2006) Multiple roles of cord factor in the pathogenesis of primary, secondary, and cavitary tuberculosis, including a revised description of the pathology of secondary disease. Ann Clin Lab Sci. 2006 Autumn;36(4):371-86.Abstract
Guidry, TV, Hunter RL, Actor, JK. (2006) CD3+ cells transfer hypersensitive granulomatous response to mycobacterial glycolipid Trehalose 6,6’-Dimycolate in mice. Microbiology-UK(SGM). Microbiology. 2006 Dec;152(Pt 12):3765-75.Abstract
Hunter RL, Olsen M, Jagannath C, Actor, JK. (2006) Trehalose 6,6'-dimycolate and lipid in the pathogenesis of caseating granulomas of tuberculosis in mice. Am J Pathol. 168(4):1249-61.Abstract
Hwang SA, Kruzel ML, Actor, JK. (2005) Lactoferrin augments BCG vaccine efficacy to generate T helper response and subsequent protection against challenge with virulent Mycobacterium tuberculosis. Int Immunopharmacol. 5(3):591-9.Abstract
Borders CW, Courtney A, Ronen K, Pilar Laborde-Lahoz M, Guidry TV, Hwang SA, Olsen M, Hunter RL Jr, Hollmann TJ, Wetsel RA, Actor, JK. (2005) Requisite role for complement C5 and the C5a receptor in granulomatous response to mycobacterial glycolipid trehalose 6,6'-dimycolate. Scand J Immunol. 2005 Aug;62(2):123-30.Abstract
Guidry TV, Olsen M,
Hwang, S-A, Kruzel, ML, Actor, JK. (2005) Lactoferrin Augments BCG Vaccine Efficacy to Generate T Helper Response and Subsequent Protection against Challenge with Virulent Mycobacterium tuberculosis. Intern Immunopharm. 5(3):591-599.Abstract
Indrigo, J, Hunter, RL Jr, Actor, JK. (2003) Cord Factor Trehalose 6,6’-dimycolate (TDM) Mediates Trafficking Events During Mycobacterial Infection of Murine Macrophages. Microbiology-UK(SGM). 149(8): 2049-2059.Abstract
Indrigo, J, Hunter, RL Jr, Actor, JK. (2002) Influence of trehalose 6,6'-dimycolate (TDM) during mycobacterial infection of bone marrow macrophages. Microbiology. 148(7): 1991-1998 Abstract
Actor, JK, Indrigo, J, Beachdel, CM, Olsen, M, Wells, A, Hunter, RL, Dasgupta, A. (2003) Mycobacterial Glycolipid Cord Factor Trehalose 6,6'-dimycolate Causes Decrease in Serum Cortisol during Granulomatous Response. NeuroImmunomodulation. 10(5):270-282 Abstract
Actor, JK, Hwang, S-A, Olsen, M, Zimecki, M, Hunter, RL, Jr., Kruzel, ML. (2002) Lactoferrin immunomodulation of DTH response in mice. International Immunopharmacology. 2:475-486 Abstract
Wang, H, Actor, JK, Indrigo, J, Olsen, M, Dasgupta, A. (2003) Asian and Siberian ginseng as a potential modulator of immune function: an in vitro cytokine study using mouse macrophages. Clin Chim Acta. 2003 Jan;327(1-2):123-128. Abstract
Kruzel, ML, Harari, Y, Mailman, D, Actor, JK, Zimecki, M. (2002) Differential effects of prophylactic, concurrent and therapeutic lactoferrin treatment on LPS-induced inflammatory responses in mice. Clinical Experimental Immunology. 130(1):25-31 Abstract
Actor, JK, Olsen, M, Hunter, RL, Jr., Geng, Y-J. (2001) Dysregulated Response to Mycobacterial Cord Factor Trehalose-6,6'-Dimycolate in CD1D-/- Mice. Journal of Interferon and Cytokine Research. 21(12):1089-96 Abstract
Actor, JK, Breij, E, Wetsel, RA, Hoffmann, H, Hunter, RL, Jr., Jagannath, C. (2001) A Role for Complement C5 in Organism Containment and Granulomatous Response during Murine Tuberculosis. Scandanavian Journal of Immunology. 53(5):464-74. Abstract
Actor, JK. (2001)
Quantitation of Cytokine mRNA by Flash-type Bioluminescence. L. O’Neill,
A. Bowie Eds;. Methods in
Molecular Medicine: Interleukin Protocols. Humana Press.
Actor, JK, Olsen, M, Jagannath, C, & Hunter, RL. (1999) Relationship between Survival, Organism Containment and Granuloma Formation in Acute Murine Tuberculosis. Journal of Interferon and Cytokine Research 19(10):1183-1193. Abstract
Actor, JK. (2000) Bioluminescent quantitation and detection of gene expression during infectious disease. Combinatorial Chemistry and High Throughput Screening. 3(4):273-288. Abstract | Article
Watson, VE, Hill, LL, Owen-Schaub,
LB,
Actor, JK, Leonard, CD, Watson, VE, M, Wells, A, Jagannath, C, Hunter, RL, Jr, and Dasgupta, A. (2000) Cortisol serum depression during murine lung inflammation induced by Mycobacterium tuberculosis and the mycobacterial cord factor trehalose-6,6'-dimycolate. J. Mol. Diag. 1999; 1(1): 52 (#ID13). Combinatorial Chemistry and High Throughput Screening. 3(4):343-351. Abstract | Article
Jagannath C, Hoffmann H, Sepulveda E, Actor, JK, Wetsel RA, Hunter RL. (2000) Hypersusceptibility of A/J mice to tuberculosis is in part due to a deficiency of the fifth complement component (C5). Scand J Immunol. Oct;52(4):369-79. Abstract
Perez, RL, Schuyler, KD, Little, C, Olsen, M, Indrigo, J, Hunter, RL Jr., Actor, JK. (2000) Cytokine Message and Protein Expression during Lung Granuloma Formation and Resoplution Induced by the Mycobacterial Cord Factor Trehalose-6,6'-Dimycolate. Journal of Interferon and Cytokine Research. 20(9):795-804. Abstract
Ozpolat, B, Actor, JK, Rao, X-M, Lee, S, Osato, M, Graham, DY, Lachman, LB. (2000) Quantitation of Helicobacter pylori in the stomach using quantitative polymerase chain reaction assays. Helicobacter. 5(1):13-21. Abstract
Actor, JK, Nolte, FS, & Smith, DF.
(1999) "AquaLite(r), the bioluminescent label
for immunoassays, gene detection, and molecular diagnostics" In: Bioluminescence
and Chemiluminescence: Perspectives for the 21st
Century, Proceedings of the 10th International Symposium on Bioluminescence and
Chemiluminescence held at
Actor, JK, Limor, JR, & Hunter, RL. (1999) A Flexible Bioluminescent-Quantitative Polymerase Chain Reaction Assay for Analysis of Competitive PCR Amplicons. J. Clin Lab Anal 13(1):40-47. Abstract
Smith DF, Stults, NL, Cormier, MJ, & Actor, JK. (1999) Recombinant Aequorin: A Bioluminescent Signal for Molecular Diagnostics. J. Clin Lig Assay 22:158172. Abstract
Actor, JK, Kuffner T, Dezzutti CS, Hunter RL, McNicholl J. (1998) A flash-type bioluminescent immunoassay that is more sensitive than radioimaging: quantitative detection of cytokine cDNA in activated and resting human cells. J Immun Meth 211:65-77. Abstract
Jagannath, C, Pai, S, Actor, JK & Hunter, RL. (1999) CRL-1072 enhances antimycobacterial activity of human macrophages through interleukin-8. J. Interferon and Cytokine Res. J. Interferon and Cytokine Res. 19(1):67-76. Abstract
Jagannath C, Actor, JK, Hunter RL. (1998) Induction of nitric oxide in human monocytes and monocyte cell lines by Mycobacterium tuberculosis. Nitric Oxide: Biology and Chemistry. 3:174-186. Abstract
Newman MJ, Actor, JK, Balasubramanian
M, Jagannath C. (1998) Use of Non-ionic Block Copolymers in Vaccines and
Therapeutics. Critical Reviews in Therapeutic Drug Carrier Systems, Vol 15(2). Plenum Press,
Siddiqi AM,
Actor, JK, Limor JR & Hunter RL. (1997) Bioluminescent detection of PCR products generated during log-phase PCR amplification: Optimization of substrate conditions. Clinical Chemistry. 43(6):s274.
Actor, JK, Marshall, MA, Eltoum, IA, Buller, RML, Berzofsky, JA, & Sher, A. (1994) Increased susceptibility of mice infected with Schistoma mansoni to recombinant vaccinia virus: association of viral persistence with egg granuloma formation. Eur. J. Immunol. 24: 3050-3056. Abstract
Actor, JK, Shirai
M, Kullberg MC, Buller RM, Sher
A, Berzofsky JA. (1993) Helminth
infection results in decreased virus-specific CD8+ cytotoxic T-cell and Th1
cytokine responses as well as delayed virus clearance. Proc Natl
Acad Sci
Giese NA, Gazzinelli RT, Actor, JK, Morawetz RA, Sarzotti M, Morse HC. (1996) Retrovirus-elicited IL-12 and TNF-a as inducers of IFN-g-mediated pathology in mouse AIDS. Immunol 87:467. Abstract
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