A Relational Database for Diagnosis of Hematopoietic Neoplasms Using Immunophenotyping by Flow Cytometry

Pathology Grand Round

6/3/98


Click here to start


Table of Contents

A Relational Database for Diagnosis of Hematopoietic Neoplasms Using Immunophenotyping by Flow Cytometry

INTRODUCTION

Basic Components of Flow Cytometry

Light Scattering

Scattergram, peripheral blood

Scattergram, lymph node

Antigen Detection

Wavelength of Fluorochromes

Chronic Lymphocytic Leukemia

A Case Illustration

Peripheral Blood Smear

Bone Marrow Aspirate

Flow Cytometry Results

Immunophenotyping by Flow Cytometry

Goal of this Study

Relational Database

A Simplified Example of Database

Database as a Decision-Support System

MATERIALS AND METHODS

Relational Database CD-Marker

Ranking Mechanism: Matching Factor (MF)

Illustration for Calculation of MF

Outline of the Demonstration Case

Methods for Validating Database CD-Marker

Methods for Validating (cont’d)

Results of Validation

Cases not successfully ranked

Cases not successfully ranked (cont’d)

Cases not successfully ranked (cont’d)

Validation Results (after revision)

DISCUSSION: Previous Work by Others

Previous Work by Others (cont’d)

Unique Features in Design of CD-Marker

WEB MARKER

Constraints in using CD-Marker

Conclusion

Self-Learning Mechanism

Author: Andy Nguyen, M.D.

Email: nguyen@casper.med.uth.tmc.edu

Home Page

Download presentation source (Powerpoint file)