Current Research Interests

This laboratory recently obtained a research grant from the National Center for Research Resources, an agency of the National Institutes of Health for development of a new class of zwitterions compounds for application in Proteomics. Briefly, the currently available zwitterions mixtures used in the first dimension of 2D electrophoresis are notoriously variable in their properties from batch to batch. This compromises many studies relying on 2D electrophoresis in the analytical phase of the studies. In collaboration with scientists from Huntsman Ethyleneamines, Ltd. and with Dr. Phil DeShong at the University of Maryland, this lab will develop further the methods for isolation of the linear form of polyethylenepolyamines in pure form prior to reaction with acid groups to create zwitterions mixtures for 2D electrophoresis. Methods for analysis of zwitterion mixtures which were patented earlier by this lab will be used to help develop the chemistry for creation of mixtures of zwitterions that do not vary in their properties from batch to batch.

The second project in the laboratory centers on biochemical and immunological studies of a soluble form of the Rh antigen from red blood cells and adhering it to solid-phase matrices. This allows us to coat plastic beads with antigen and to purify anti-Rh antibodies by affinity chromatography. Further, we can coat wells on 96-well plastic plates and do quantitative ELISA analysis of anti-Rh antibodies eliminating the doubling-dilution endpoint estimation of "titers" which are notoriously inaccurate and insensitive ways of determining levels of antibodies.

Recent publications:

Yared, M. A., K. J. Moise, Jr., and L. S. Rodkey. 1997. Stable Solid-Phase Rh Antigen. Transfusion Medicine. 7:311-317

Gololobov, G. V., Rumbley, C. A., Rumbley, J. N., Schourov, D. V., Makarevich, O. I., Gabibov, A. G., Voss, E. W. and Rodkey, L. S. 1997. DNA hydrolysis by monoclonal anti-ssDNA autoantibody BV 04-01: Origins of catalytic activity. Molecular Immunology. 34:1083-1093.

Moise, K. J., Rodkey, L. S., Yared, M., Hudon, L., Saade, G., Dorman, K. and Graham, A. 1998. An animal model for hemolytic disease of the fetus/newborn in New Zealand White and New Zealand Red rabbits: Newborn effects. American Journal of Obstetrics and Gynecology. 170:1353-1358.

Rodkey, L. S. and Tissot, R. G. 1998. Rabbit blood groups. in Handbook of Vertebrate Immunology. p. 242-243. Eds. P.-P. Pastoret, P. Griebel, H. Bazin, & A. Govaerts. Academic Press Limited.

Rodkey, L. S. and Nisonoff, A. 1999. Haptens. In: Encyclopedia of Life Sciences. London. Nature Publishing Group. http://www.els.net

Rodkey, L. S., Rumbley, C. A., Rumbley, J. N., Schourov, D. V., Makarevich, O. I., Gabibov, A. G. and Voss, E. W. 2000. DNA hydrolysis by monoclonal antibody BV 04-01. Applied Biochemistry and Biotechnology. 83:95-105.

Patents

Abridged Curriculum Vitae

Ampholife Technologies, Inc.